梓醇对大鼠慢性脑缺血白质损伤的保护作用

被引：10

作者：

蔡其燕

陈兴书

姚忠祥

机构：

[1] 第三军医大学基础医学部组织学与胚胎学教研室,重庆市神经科学研究所

来源：

第三军医大学学报 | 2010年 / 32卷 / 21期

关键词：

梓醇; 脑缺血; 髓鞘; 白质; 大鼠;

D O I：

10.16016/j.1000-5404.2010.21.002

中图分类号：

R285.5 [中药实验药理];

学科分类号：

100806 [中药药理学];

摘要：

目的初步探讨梓醇对慢性脑缺血所致白质损伤的保护作用及机制。方法成年雄性Wistar大鼠24只,分为4组:假手术组,缺血组,生理盐水组和梓醇组。通过结扎大鼠双侧颈总动脉建立慢性脑缺血模型,同时腹腔注射5mg/kg梓醇或生理盐水予以治疗,每天1次,连续10d。术后30d处死,然后利用卢卡斯快蓝(luxol fast blue,LFB)染色法和免疫组织化学技术分别观察梓醇对脑白质髓鞘以及髓鞘碱性蛋白(myelin basic protein,MBP)、腺瘤性息肉蛋白(adenoma-tus polyposis coli,APC(CC-1)和星形胶质纤维酸性蛋白(glial fibrillary acidity protein,GFAP)表达的影响。结果与假手术组比较,在缺血组和生理盐水组,LFB染色变浅[(37259.6±6626.6)、(36554.4±7865.7)vs(90470.5±5004.6)],MBP表达下降[(24016.5±3794.8)、(23923.6±4605.1)vs(65055.4±6839.9)],CC-1阳性的少突胶质细胞(oligo-dendrocytes,OLs)减少[(33.0±9.6)、(32.0±9.8)vs(100.0±11.2)],GFAP阳性的星形胶质细胞增多[(52.0±7.4)、(50.0±9.4)vs(14.0±6.6)],均有显著差异(P<0.01);梓醇治疗后能显著地减轻白质损伤,它能使LFB染色加深(86687.6±6619.1),MBP表达升高(61604.5±6319.9),CC-1阳性细胞数增加(91.0±8.4),GFAP阳性细胞数减少(17.0±7.8),与生理盐水组比较均有显著差异(P<0.01)。结论梓醇可通过抑制星形胶质细胞的增生进而抑制炎症反应来保护慢性脑缺血所致的白质损伤。

引用

页码：2273 / 2276

页数：4

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