从山东省家犬血液中分离出致病性巴尔通体——文森巴尔通体伯格霍夫亚种

被引：12

作者：

栗冬梅 ^{[1
]}

孟凤霞 ^{[1
]}

宋秀平 ^{[1
]}

秦增军 ^{[2
]}

杨小冉 ^{[1
]}

吴海霞 ^{[1
]}

任东升 ^{[1
]}

刘起勇 ^{[1
]}

机构：

[1] 中国疾病预防控制中心传染病预防控制所

[2] 山东省阳谷县畜牧局

来源：

中华流行病学杂志 | 2006年 / 04期

关键词：

巴尔通体; 家犬; 序列分析; 菌血症;

D O I：

暂无

中图分类号：

S858.292 [犬];

学科分类号：

0906 ;

摘要：

目的调查家犬巴尔通体的带菌状况,分离培养并鉴定菌种。方法将采获的家犬血标本抗凝血接种于含5％去纤维兔血的脑心浸液培养基上,置于37℃含5％CO2培养箱中分离巴尔通体。然后挑选巴尔通体疑似菌落染色镜检,应用聚合酶链反应技术(PCR)在属分类水平鉴定巴尔通体,通过PCR产物限制性片段长度多态性分析(PCR-RFLP)方法在分离菌株及与阳性对照菌株之间鉴别。选择16S rRNA、gltA和16S-23S rRNA ITS的PCR产物测序,将所测核酸序列进行同源性比较及系统发育分析确定巴尔通体种或基因型。结果从山东省采获的71份犬血液中分离培养出 2株巴尔通体疑似菌株,光镜下观察为革兰染色阴性、微弯曲的细小杆菌,3对巴尔通体属特异性引物扩增结果阳性,PCR-RFLP分析2株分离菌株相同,与阳性对照不同,16S rRNA、gltA和16S-23S rRNA ITS序列分析结果表明2株巴尔通体分离株与文森巴尔通体伯格霍夫亚种的同源性分别为 100．0％、99．7％和97．2％。结论山东省家犬中存在巴尔通体感染,分离培养出的菌株经鉴定为文森巴尔通体伯格霍夫亚种,该亚种属于致病性巴尔通体。

引用

页码：333 / 338

页数：6

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