乙醇酸氧化酶的纯化结晶和酪氨酸残基的修饰对酶活性的影响

被引：11

作者：

彭新湘

李明启

机构：

[1] 华南农业大学农业生物系光合作用研究室,华南农业大学农业生物系光合作用研究室广州,广州

来源：

植物生理学报 | 1989年 / 03期

关键词：

乙醇酸氧化酶; 化学修饰; N—溴代琥珀酰亚胺; 对硝基苯磺酰氟; N—乙酰咪唑;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

从青菜叶片中纯化的GAO为淡黄色棒状或针状结晶,纯化度达129.3倍。NBS对GAO有强烈的抑制作用,其抑制效应不受pH的影响,抑制程度和 NBS浓度（0～10 μmol/L）呈线性关系;乙醇酸和草酸均能保护GAO免受NBS的抑制。NBS在全抑制浓度范围内并不降低OD280,但引起OD260明显上升;当用乙醇酸保护GAO时,这种上升与酶的抑制同时消失。另外两种更专一的酪氨酸残基修饰剂NBSF和N—AI亦显著抑制GAO活性,底物乙醇酸对此有保护作用,N—AI抑制的GAO可被羟胺复活。

引用

页码：257 / 262

页数：6

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