DNA聚合酶-ⅠKlenow片段原位检测缺血性神经元的DNA早期断裂

被引：3

作者：

徐若华

万琪

王洪典

李兵

张巍

机构：

[1] 第四军医大学西京医院神经内科!陕西西安

[2] 第四军医大学西京医院神经外科!陕西西安

来源：

中华老年心脑血管病杂志 | 2000年 / 04期

关键词：

脑缺血; DNA损伤; Klenow片段;

D O I：

暂无

中图分类号：

R743.3 [急性脑血管疾病（中风）];

学科分类号：

1002 ;

摘要：

目的　探讨脑缺血 30min后DNA断裂损伤的发生时间。方法　用DNA聚合酶 ⅠKlenow大片段原位检测缺血性神经元的DNA早期断裂。结果　缺血再灌注 1h顶叶皮层、梨状皮层出现DNA断裂 ,再灌注 2h尾壳核出现DNA断裂 ,再灌注 2 4h ,各个区域的DNA断裂均达到顶峰。结论　DNA断裂损伤可能是缺血神经元改变的早期事件之一。

引用

页码：266 / 268

页数：3

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