即刻早期基因c-fos,c-jun在缺血再灌注大鼠肝脏中的表达

被引：9

作者：

李广罡

韩文斌

陈飞

机构：

[1] 北京军区总医院重症监护科

来源：

中国热带医学 | 2010年 / 10卷 / 09期

关键词：

肝脏; 缺血再灌注损伤; 细胞凋亡; 即刻早期基因(immediate early genes,IEGs);

D O I：

暂无

中图分类号：

R363 [病理生理学];

学科分类号：

100103 [病原生物学];

摘要：

目的研究即刻早期基因家族中c-fos、c-jun基因在缺血再灌注肝脏中的表达情况,探讨其与缺血再灌注损伤发生的关系。方法 SD大鼠随机分为假手术组、缺血再灌注后30min组和缺血再灌注后120min组。全自动生化分析仪测定血清ALT、AST水平,光镜观察肝组织形态学改变,TUNEL法测定肝细胞凋亡指数。实时荧光定量PCR(realtimePCR)法测定组织中c-fos及c-jun的表达情况。结果肝脏缺血再灌注损伤后血清ALT、AST逐步上升(P<0.05)。病理改变显著,细胞凋亡现象逐步显现。c-fos及c-jun的表达水平,在再灌注后30min明显升高(P<0.05),再灌注2h后表达水平有所回落,但仍显著高于假手术组(P<0.05)。结论 c-fos及c-jun基因参与肝脏缺血再灌注损伤的发生,并可能与肝细胞凋亡与再生的过程相关。

引用

页码：1048 / 1049

页数：2

共 6 条

[1]

Reperfusion stress induced during intermittent selective clamping accelerates rat liver regeneration through JNK pathway [J].

Duval, Helene ;

Mbatchi, Sasse-Fanie ;

Grandadam, Stephane ;

Legendre, Claire ;

Loyer, Pascal ;

Ribault, Catherine ;

Piquet-Pellorce, Claire ;

Guguen-Guillouzo, Christiane ;

Boudjema, Karim ;

Corlu, Anne .

JOURNAL OF HEPATOLOGY, 2010, 52 (04) :560-569

[2]

Genomewide expression profiles of rat model renal isografts from brain dead donors [J].

Kusaka, Mamoru ;

Kuroyanagi, Yoko ;

Kowa, Hiroe ;

Nagaoka, Kayuri ;

Mori, Terumi ;

Yamada, Kouji ;

Shiroki, Ryoichi ;

Kurahashi, Hiroki ;

Hoshinaga, Kiyotaka .

TRANSPLANTATION, 2007, 83 (01) :62-70

[3]

Spatio-temporal expression of immediate early genes in the central nervous system of SJL/J mice [J].

Gerhauser, I ;

Alldinger, S ;

Ulrich, R ;

Baumgärtner, W .

INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE, 2005, 23 (07) :637-649

[4]

Effects of preconditioning on ischemia/reperfusion injury of hepatocytes determined by immediate early gene transcription.[J].Shunnichi Ishii;Tsuyoshi Abe;Takuro Saito;Takao Tsuchiya;Hirotaka Kanno;Masatsugu Miyazawa;Masao Suzuki;Ryouichi Motoki;Mitsukazu Gotoh.First Department of Surgery; School of Medicine; Fukushima Medical University; Fukushima 960-1295; Japan.2001, 5

[5]

ENDOTHELIAL CELL AND HEPATOCYTE DEATHS OCCUR BY APOPTOSIS AFTER ISCHEMIA-REPERFUSION INJURY IN THE RAT LIVER1;2.[J].Vivek Kohli;Markus Selzner;John F. Madden;Rex C. Bentley;Pierre-Alain Clavien.Transplantation.1999, 8

[6]

Preconditioning effects on expression of proto-oncogenes c-fos and c-jun after hepatic ischemia/reperfusion in rats [J].

JianSheng Xiao FangGang Cai Ying Niu Yi Zhang XianLing Xu and QiFa Ye Wuhan China Research Institute of Organ Transplantation Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China ;

Department of General Surgery First Affiliated Hospital Fujian Medi cal College Fuzhou China ;

and Xiangya Medical Trans plantation Academy of Central South University Changsha China .

Hepatobiliary & Pancreatic Diseases International, 2005, (02) :197-202

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