抗高浓度葡萄糖阻遏的纤维素酶高产菌的选育

被引:15
作者
宋小炎
宋桂经
孙彩云
刘陶陶
韩峰
机构
[1] 山东大学微生物技术国家重点实验室!济南
关键词
纤维素酶; 抗阻遏; 葡萄糖;
D O I
暂无
中图分类号
Q933 [微生物遗传学];
学科分类号
071007 ;
摘要
以本室保存的一株纤维素酶产量较高的拟康氏木霉为出发菌株,经紫外诱变处理,用葡萄糖( 梯度浓度) 纤维素双层平板选育,在含葡萄糖(4% ) 纤维素双层平板上获得一株葡萄糖浓度高达10% 仍能产生纤维素水解透明圈的突变株UVⅢ.培养6 d ,干曲的CMC 酶活、FP 酶活和βG 酶活分别可达1145 .7 u/g 、55 .6 u/g 和24 u/g .分别是出发株的2 .4 倍、3.4 倍和12 .6 倍.
引用
收藏
页码:488 / 492
页数:5
相关论文
共 8 条
[1]   MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF THE CELLOBIOHYDROLASE-I GENE FROM TRICHODERMA-KONINGII-G-39 [J].
WEY, TT ;
HSEU, TH ;
HUANG, L .
CURRENT MICROBIOLOGY, 1994, 28 (01) :31-39
[2]  
Cloning of a Gene Encoding a Putative Carbon Catabolite Repressor from Trichoderma reesei. Shou Takashima,Akita Nakamuta,Hiroshilikuta,Haruhiko Masaki and Takeshi Uozmi. Bioscience Biotechnology and Biochemistry . 1996
[3]  
Enfanced cellulase production by a mutantof Trichoderma reesei. Gadgilo NJ,Daginawala HF,Chakrabarti T,and Khanna P. En Microb Technol . 1995
[4]  
Sophorose as aninducerofcellulasein Trichoderma Veride ,J. Mandels M,Parrish F W and Reese E. Bact . 1962
[5]  
Prepartion lf Mutants Resistant toCatabolite Repression of Trichoderma reesei. Kawamori M,Morikawa Y,Shinsha Y,Takayama K and Takasawa S. Agricultural and Biological Chemistry . 1985
[6]   INDUCTION AND PRODUCTION OF CELLULASES BY L-SORBOSE IN TRICHODERMA-REESEI [J].
KAWAMORI, M ;
MORIKAWA, Y ;
TAKASAWA, S .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1986, 24 (06) :449-453
[7]  
A Constritutively Cellulases - Producing Mutantof Trichoderma reesei. Misara S,Gopalkrisnan KSand Ghose TK. Biotechnology and Bioengineering . 1982
[8]  
Induction of cellulase formation in Trichoderma reesei by cellobiono-1,5-lacton[J] . C. B. Iyayi,E. -E. Bruchmann,C. P. Kubicek. &nbspArchives of Microbiology . 1989 (4)