脂肪肝病变大黄鱼肝脏脂肪酸组成、代谢酶活性及抗氧化能力的研究

被引：17

作者：

曾端

麦康森

艾庆辉

机构：

[1] 中国海洋大学教育部海水养殖重点实验室

来源：

中国海洋大学学报(自然科学版) | 2008年 / 04期

关键词：

大黄鱼; 脂肪肝; 脂肪酸; 酶; MDA;

D O I：

10.16441/j.cnki.hdxb.2008.04.005

中图分类号：

S943 [各种鱼的病害、敌害及其防治];

学科分类号：

摘要：

脂肪肝病变在人工养殖大黄鱼(Pseudosciaena crocea)中非常普遍,本试验研究了大黄鱼脂肪肝的发生机制,为寻找解决大黄鱼脂肪肝的有效途径提供基础资料。试验鱼取自中国东海,养殖方式和饲料来源相同,根据肉眼和组织学观察分为正常肝脏、轻微脂肪肝和严重脂肪肝3类。分别测定了正常大黄鱼和具有脂肪肝症状的大黄鱼肝脏总脂脂肪酸组成的变化、相应脂肪酸合成酶、抗氧化酶以及丙二醛含量的变化。试验结果显示,随着大黄鱼脂肪肝症状严重程度增加,肝脏总脂饱和脂肪酸和n-3多不饱和脂肪酸比例显著降低(P<0.05),单不饱和脂肪酸比例显著上升(P<0.05)。其中严重脂肪肝花生四烯酸(ARA)、二十碳五烯酸(EPA)、二十二碳六烯酸(DHA)比例较正常鱼分别降低了89%,79%和78%。严重脂肪肝大黄鱼的异柠檬酸脱氢酶和6-磷酸葡萄糖脱氢酶活性显著高于正常鱼(P<0.05);轻微脂肪肝鱼的转氨酶活性显著低于正常鱼(P<0.05);具有脂肪肝症状的大黄鱼超氧化物歧化酶和过氧化氢酶活性均高于正常鱼。丙二醛含量则随大黄鱼脂肪肝病变程度加剧而显著增加(P<0.05)。通过分析认为,过量单不饱和脂肪酸沉积在肝脏中,导致脂肪酸合成代谢发生紊乱,可能是造成大黄鱼脂肪肝的主要原因;肝脏ARA含量大量减少使脂肪肝大黄鱼抗应激能力降低,而肝脏脂肪酸过氧化程度升高,进一步破坏肝脏的正常功能。

引用

页码：542 / 546

页数：5

共 14 条

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