肝癌亚细胞结构的蛋白质组分比较分析

被引：3

作者：

李兴 ^{[1
]}

潘卫 ^{[2
]}

邱峰 ^{[3
]}

邱宗荫 ^{[1
]}

机构：

[1] 重庆医科大学医学检验系

[2] 贵阳医学院医学检验系

[3] 重庆医科大学药学系

来源：

分子细胞生物学报 | 2006年 / 05期

关键词：

肝癌细胞; 亚细胞蛋白质组; 线粒体; 细胞核;

D O I：

暂无

中图分类号：

R735.7 [肝肿瘤];

学科分类号：

摘要：

运用亚细胞蛋白质组学的研究策略,分离纯化亚细胞结构,可以提高低丰度蛋白质在双向电泳中检出的数量。通过对比分析肝癌细胞与正常肝细胞线粒体、细胞核蛋白质组的差异表达情况,为肝癌发病机理的研究提供更多、更有价值的信息。以体外培养的人体肝癌细胞QGY-7703与正常肝细胞LO2为研究模型,通过超离心的方法分离细胞的线粒体和细胞核。双向电泳分离线粒体和细胞核的蛋白质,图像分析筛选差异表达蛋白斑点,MALDI-TOF-MS鉴定蛋白质。从线粒体、细胞核的蛋白质电泳图谱中筛选出54个候选差异表达的蛋白质斑点,质谱鉴定出22种差异表达蛋白质,其中17种在肝癌细胞中表达上调,5种在肝癌细胞中表达下调。筛选出的差异表达蛋白质涉及到细胞的能量代谢、蛋白质合成、细胞骨架与核骨架的改变、mRNA的加工成熟及凋亡调控等许多方面,表明癌变细胞的组织结构和代谢状态都发生了很大的变化。

引用

页码：399 / 406

页数：8

共 18 条

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