转基因大豆对根际固氮细菌群落多样性的影响

被引:10
作者
赖欣
张永生
赵帅
李刚
杨殿林
机构
[1] 农业部环境保护科研监测所,农业部转基因生物生态环境安全监督检验测试中心
关键词
转基因大豆; 土壤细菌多样性; 固氮细菌; nifH; DGGE;
D O I
10.13292/j.1000-4890.2010.0315
中图分类号
S154.3 [土壤微生物学];
学科分类号
071012 ; 0713 ;
摘要
为了研究种植转基因大豆在土壤生态系统中对固氮细菌的影响,应用PCR-DGGE技术分析种植不同大豆处理下土壤细菌固氮酶nifH基因的分子多样性。结果表明:低GC含量的厚壁菌门出现在DGGE图谱的上部,而且受生育时期影响比较明显;绿菌门相对保守,不受生育时期和种植品种的影响;在测序得到的46个结果中,共计28个属于变形菌门,占60.8%,广泛分布于DGGE胶的各个部位,大部分较稳定,不受种植品种或者采样时间的影响。相对于不同生育时期对根际土壤固氮细菌的影响,种植转基因大豆、亲本非转基因大豆和普通大豆之间的差异并不明显,表明种植转基因大豆对土壤固氮细菌没有明显的影响。
引用
收藏
页码:1736 / 1742
页数:7
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