IL-17对人牙周膜成纤维细胞RANKL表达的影响

被引：6

作者：

林丹萍

王威栋

李璐

叶宇

孙颖

徐艳

机构：

[1] 南京医科大学口腔医学研究所,南京医科大学附属口腔医院牙周科

来源：

口腔医学 | 2014年 / 34卷 / 06期

关键词：

牙周膜成纤维细胞; 白细胞介素17; 细胞核因子κB受体活化因子配体;

D O I：

10.13591/j.cnki.kqyx.2014.06.002

中图分类号：

R780.2 [口腔病理学];

学科分类号：

100301 ;

摘要：

目的观察IL-17对人牙周膜成纤维细胞RANKL表达的影响,探讨IL-17调控牙周炎骨改建的可能致病机制。方法用不同浓度(0、10、25、50 ng/mL)的IL-17处理人牙周膜成纤维细胞。选择最佳刺激浓度,用该浓度的IL-17处理细胞不同时间(0、12、24 h)。通过实时定量RT-PCR和Western-blot的方法检测细胞中骨吸收相关因子RANKL的表达水平。结果 IL-17可显著提高人牙周膜成纤维细胞RANKL的表达水平,并随着浓度的提高和刺激时间的延长而增加。结论IL-17可上调人牙周膜成纤维细胞的RANKL表达,提示IL-17可通过调控人牙周膜成纤维细胞骨吸收相关因子的表达参与牙槽骨的改建。

引用

页码：405 / 408

页数：4

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