白藜芦醇预处理对神经干细胞氧糖剥夺损伤的保护作用

被引:6
作者
韩宁
殷安安
章翔
王江
叶玉勤
陈晓燕
机构
[1] 第四军医大学西京医院神经外科
关键词
白藜芦醇; 神经干细胞; 氧糖剥夺; 细胞活性; 乳酸脱氢酶;
D O I
暂无
中图分类号
R965 [实验药理学];
学科分类号
100706 [药理学];
摘要
目的探讨白藜芦醇预处理对神经干细胞氧糖剥夺损伤的保护作用。方法用含有白藜芦醇的细胞培养基预培养神经干细胞1 h,更换低糖培养基后将细胞培养在缺氧培养盒内6 h进行氧糖剥夺实验(OGD);采用噻唑蓝(MTT)比色实验检测细胞活性;用LDH试剂盒测细胞培养基中乳酸脱氢酶(LDH)活性。结果白藜芦醇预处理(PRC)组细胞MTT值高于对照组(n=7,P<0.01),PRC组细胞培养基上清中LDH低于对照组(n=5,P<0.01)。结论白藜芦醇预处理对神经干细胞氧糖剥夺损伤有保护作用。
引用
收藏
页码:23 / 26
页数:4
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