液滴数字PCR在肿瘤个体化治疗方面的应用

被引:9
作者
王青 [1 ]
刘宝瑞 [2 ]
机构
[1] 南京医科大学鼓楼临床医学院
[2] 南京大学医学院附属鼓楼医院
关键词
个体化治疗; 肿瘤; 液滴数字PCR;
D O I
暂无
中图分类号
R730.5 [肿瘤治疗学];
学科分类号
摘要
个体化治疗可以提高肿瘤病人的生存质量和生存时间,而肿瘤患者基因状态指导个体化用药,商业化的液滴数字PCR(droplet digital PCR,dd PCR)已经上市,并且可以在一个样本中对核酸精确定量。它是通过将模板稀释到20000个水包油微滴实现的,每个液滴可被微滴阅读器判定为阳性或阴性,因此某个等位基因的浓度可以被精确计算,它能够用于检测稀有突变体以及定量。与传统的实时PCR(q PCR)相比,dd PCR具有许多优点,尤其是灵敏且能够获得精确定量,例如,可以检测石蜡包埋样品中基因组扩增;可以以非侵入性的方式通过体液,如血液和痰来检测肿瘤基因突变;也可通过dd PCR检测拷贝数变异,从而研究其与癌症的相关性。
引用
收藏
页码:1771 / 1774
页数:4
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