基于核酸适配体与银纳米粒子探针共振光散射技术检测卡那霉素

被引：4

作者：

廖且根

李伟红

张金艳

罗林广

机构：

[1] 江西省农业科学院农产品质量安全与标准研究所,江西省农业科学院农产品质量安全重点实验室

来源：

农产品质量与安全 | 2012年 / S1期

关键词：

共振光散射; 核酸适配体; 银纳米粒子; 卡那霉素;

D O I：

暂无

中图分类号：

S859.84 [动物性食品中化学物残留];

学科分类号：

0906 ;

摘要：

本文建立了一种新型灵敏的基于未修饰的银纳米粒子和卡那霉素适配体为探针共振光散射分析卡那霉素方法。单链DNA能缠绕在银纳米粒子表面,使其即使在高盐浓度下仍不发生聚集。当卡那霉素及其适配体相互作用后,适配体的构象发生变化,不能吸附在粒子表面,在盐存在下,银纳米粒子聚集,产生增强的散射信号。在优化的条件下,散射信号与卡那霉素在2.0nmol/L到20.0nmol/L浓度范围内呈线性关系,检测限为1.0μg/kg。适配体相对其他的抗生素显示出高亲和性,能够应用于鸡肉基质的卡那霉素残留分析。

引用

页码：17 / 20

页数：4

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