黄芪甲苷对脑缺血/再灌注损伤大鼠细胞自噬的影响

被引：51

作者：

李媛 ^{[1
]}

靳晓飞 ^{[2
]}

周晓红 ^{[2
]}

高维娟 ^{[2
]}

机构：

[1] 承德医学院病理生理学教研室

[2] 河北中医学院河北省心脑血管病中医药防治重点实验室

来源：

中国病理生理杂志 | 2018年 / 34卷 / 11期

关键词：

黄芪甲苷; 缺血/再灌注损伤; 自噬; 脑梗死;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理];

学科分类号：

100806 [中药药理学];

摘要：

目的:探讨黄芪甲苷对脑缺血/再灌注损伤大鼠细胞自噬的影响。方法:选取清洁级雄性SD大鼠70只随机分为假手术组、脑缺血/再灌注组、溶剂对照组、黄芪甲苷组、黄芪甲苷+自噬抑制剂组、自噬抑制剂组和自噬激活剂组。采用线栓法建立大鼠局灶性脑缺血/再灌注损伤模型。观察大鼠神经缺损症状,根据Zea Longa评分标准挑选模型成功的大鼠;采用TTC染色法检测大鼠脑梗死体积;尼氏染色观察大鼠神经细胞形态学变化;透射电子显微镜观察细胞自噬现象;Western blot检测beclin-1和LC3-Ⅱ蛋白表达量的变化。结果:假手术组无神经缺损症状,未出现脑梗死灶,尼氏体丰富、染色均匀。与假手术组相比,脑缺血/再灌注组出现明显的脑梗死灶,神经细胞坏死增多,尼氏体数量减少、着色较浅,透射电镜下可见典型的自噬体,自噬标志蛋白beclin-1和LC3-Ⅱ表达增加(P <0. 05)。与脑缺血/再灌注组相比,黄芪甲苷组和自噬激活剂组可明显减小脑梗死体积,神经细胞有不同程度的恢复,尼氏体稍增多,自噬体数量、beclin-1和LC3-Ⅱ表达量均增加(P <0. 05);自噬抑制剂组脑梗死体积增大,神经细胞坏死严重,尼氏体减少,着色变浅,自噬体数量、beclin-1和LC3-Ⅱ表达量均减少(P <0. 05);溶剂对照组无明显变化。与黄芪甲苷组比较,黄芪甲苷+自噬抑制剂组和自噬抑制剂组脑梗死体积增加,神经细胞坏死增多,尼氏体数量减少,自噬体数量、beclin-1和LC3-Ⅱ表达量降低(P <0. 05)。结论:黄芪甲苷可通过激活自噬而减轻脑缺血/再灌注损伤,从而发挥神经保护作用。

引用

页码：2037 / 2042

页数：6

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