高粱SSR分子连锁图谱的构建

被引：1

作者：

段永红 ^{[1
,2
]}

孙毅 ^{[2
]}

仪治本 ^{[3
]}

钱锦 ^{[2
]}

机构：

[1] 山西农业大学农学院

[2] 山西省农业生物技术研究中心山西省农业科学院

[3] 中北大学化工与环境学院

来源：

山西农业大学学报(自然科学版) | 2009年 / 29卷 / 04期

关键词：

高粱; SSR; 连锁图谱;

D O I：

10.13842/j.cnki.issn1671-8151.2009.04.012

中图分类号：

S514.03 [];

学科分类号：

0901 ;

摘要：

选用高粱品种B2V4×1383-2杂交组合获得的F2分离群体(共150个个体)为材料,以SSR标记构建高粱的连锁图谱。通过对129对Xtxp型SSR引物组合进行筛选,共得到75对多态性引物,利用筛选出的75对引物进行选择性扩增,得到了75个SSR多态性位点。经Map maker/EXP(version 3.0)软件处理,构建了包含12个连锁群,46个遗传标记的连锁图谱,该图谱覆盖443.9 cM,平均图距为9.6 cM。

引用

页码：315 / 319

页数：5

共 14 条

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