获取全长cDNA若干方法的比较

被引：28

作者：

陶爱林

林兴华

张端品

机构：

[1] 华中农业大学作物遗传改良国家重点实验室,华中农业大学作物遗传改良国家重点实验室,华中农业大学作物遗传改良国家重点实验室武汉,武汉,武汉

来源：

武汉植物学研究 | 2003年 / 02期

关键词：

全长cDNA; 信使RNA; 5′帽子结构; 高保真PCR; 比较;

D O I：

暂无

中图分类号：

Q522 [核糖（醣）核酸（RNA）];

学科分类号：

070307 [化学生物学];

摘要：

Full length complementary DNAs (cDNA) are crucial for the functional annotation of genes. Several novel experimental approaches for selective enrichment of full length cDNAs are outlined in parallels. Some kinds of reverse transcriptases and high fidelity PCR enzymes are introduced for promising high efficiency of accurate full length cDNA synthesis. The temperature profiles of the reverse transcription and PCR are also discussed.

引用

页码：179 / 186

页数：8

共 6 条

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