C-反应蛋白对脂肪细胞脂联素表达的影响

被引：2

作者：

黄鹏

孙玉倩

高萍

机构：

[1] 哈尔滨医科大学附属二院内分泌科

来源：

生物学杂志 | 2009年 / 26卷 / 01期

关键词：

C-反应蛋白; 脂联素; 脂肪细胞;

D O I：

暂无

中图分类号：

R341 [];

学科分类号：

1001 ;

摘要：

通过体外培养脂肪细胞,研究C-反应蛋白(CRP)对大鼠脂肪细胞脂联素蛋白分泌及mRNA基因表达的影响。取大鼠附睾脂肪垫培养脂肪细胞。用0、10、50μg/mL的CRP刺激脂肪细胞6 h,提取细胞RNA,用实时荧光定量RT-PCR技术检测脂联素mRNA表达的变化;收集细胞培养液,运用Western blot技术检测脂联素蛋白分泌的变化。结果显示0、10、50μg/mL的CRP对大鼠脂肪细胞脂联素mRNA表达的影响无差异(P>0.05)。50、10μg/mL的CRP均可减少大鼠脂肪细胞培养液中脂联素蛋白的分泌量(P<0.05)。CRP可呈剂量依赖性的降低脂肪细胞脂联素蛋白分泌的水平。而各组CRP未能影响脂肪细胞脂联素mRNA的表达。CRP对脂肪细胞脂联素基因表达和蛋白分泌的研究可以揭示转录后的控制决定了CRP对脂联素的影响。

引用

页码：8 / 10

页数：3

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