天麻素调控癫痫大鼠大脑皮质凋亡因子的作用研究

被引：32

作者：

边立功 ^{[1
]}

毕秀梅 ^{[2
]}

艾青龙 ^{[2
]}

郭家智 ^{[1
,3
]}

董书勤 ^{[1
,4
]}

许金美 ^{[1
,4
]}

钟莲梅 ^{[2
]}

陆地 ^{[1
,3
]}

机构：

[1] 昆明医科大学人体解剖学与组织胚胎学系

[2] 昆明医科大学第一附属医院神经内科

[3] 昆明医科大学生物医学工程研究中心

[4] 昆明医科大学

来源：

神经解剖学杂志 | 2016年 / 32卷 / 01期

关键词：

天麻素; 癫痫; 凋亡因子; Bcl-2; Caspase-3; 大鼠;

D O I：

10.16557/j.cnki.1000-7547.2016.01.007

中图分类号：

R285.5 [中药实验药理];

学科分类号：

100806 [中药药理学];

摘要：

目的:探讨天麻素对氯化锂-匹罗卡品诱导癫痫大鼠模型中大脑皮质神经元凋亡因子表达的作用及其机制,为临床应用天麻素治疗癫痫提供分子生物学依据。方法:80只雄性SD大鼠随机分为5组:即对照组、模型组、60 mg/kg天麻素预治疗组、120 mg/kg天麻素预治疗组和180 mg/kg天麻素预治疗组。采用氯化锂-匹罗卡品联合制备癫痫模型,治疗组给予不同剂量的天麻素预处理,模型组给以相同剂量的生理盐水。按Racine分级标准观察行为学变化,记录大发作潜伏期时间,于注射匹罗卡品后2 h给予安定终止;3 d后取材,免疫组织化学染色、免疫荧光染色和Western Blot检测凋亡相关蛋白表达变化。结果:天麻素治疗组较模型组,癫痫发作潜伏期延长,持续时间明显减少,程度减轻,死亡率显著降低,Bcl-2表达增加,Caspase-3显著降低。结论:天麻素可减少癫痫模型大鼠发作持续时间和程度,降低癫痫大鼠死亡率;天麻素通过增加癫痫大鼠大脑皮质Bcl-2和降低Caspase-3的表达而抑制凋亡,发挥脑保护作用。

引用

页码：37 / 43

页数：7

共 19 条

[1]

Acylated ghrelin protects hippocampal neurons in pilocarpine-induced seizures of immature rats by inhibiting cell apoptosis [J].

Zhang, Ruiyun ;

Yang, Guanglu ;

Wang, Qingyi ;

Guo, Feng ;

Wang, Hua .

MOLECULAR BIOLOGY REPORTS, 2013, 40 (01) :51-58

[2]

The effects of simvastatin on hippocampal caspase-3 and Bcl-2 expression following kainate-induced seizures in rats [J].

Sun, Jiahang ;

Xie, Chuncheng ;

Liu, Wei ;

Lu, Dunyue ;

Qiao, Weidong ;

Huang, Qi ;

Huo, Zhihui ;

Shen, Hong ;

Lin, Zhiguo .

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2012, 30 (04) :739-746

[3]

Kainic acid dose affects delayed cell death mechanism after status epilepticus.[J].Daisuke Tokuhara;Satoru Sakuma;Hideji Hattori;Osamu Matsuoka;Tsunekazu Yamano.Brain and Development.2006, 1

[4]

Involvement of Caspase‐3‐Like Protease in the Mechanism of Cell Death Following Focally Evoked Limbic Seizures.[J].David C.Henshall;JunChen;Roger P.Simon.Journal of Neurochemistry.2003, 3

[5]

Pilocarpine-induced status epilepticus increases cell proliferation in the dentate gyrus of adult rats via a 5-HT 1A receptor-dependent mechanism.[J].Jason J Radley;Barry L Jacobs.Brain Research.2002, 1

[6]

Alterations in bcl-2 and caspase gene family protein expression in human temporal lobe epilepsy [J].

Henshall, DC ;

Clark, RSB ;

Adelson, PD ;

Chen, M ;

Watkins, SC ;

Simon, RP .

NEUROLOGY, 2000, 55 (02) :250-257

[7]

Gene therapies that enhance hippocampal neuron survival after an excitotoxic insult are not equivalent in their ability to maintain synaptic transmission [J].

Dumas, TC ;

McLaughlin, JR ;

Ho, DY ;

Lawrence, MS ;

Sapolsky, NM .

EXPERIMENTAL NEUROLOGY, 2000, 166 (01) :180-189

[8]

Limitations in the neuroprotective potential of gene therapy with Bcl-2 [J].

Phillips, RG ;

Lawrence, MS ;

Ho, DY ;

Sapolsky, RM .

BRAIN RESEARCH, 2000, 859 (02) :202-206

[9]

Intracerebral injection of caspase-3 inhibitor prevents neuronal apoptosis after kainic acid-evoked status epilepticus [J].

Kondratyev, A ;

Gale, K .

MOLECULAR BRAIN RESEARCH, 2000, 75 (02) :216-224

[10]

MAMMALIAN CASPASES: Structure; Activation; Substrates; and Functions During Apoptosis.[J].William C. Earnshaw;Luis M. Martins;Scott H. Kaufmann.Annual Review of Biochemistry.1999,

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