银杏酸的高效液相色谱法测定

被引：23

作者：

仰榴青

吴向阳

陈钧

机构：

[1] 江苏大学药学院,江苏大学化学化工学院,江苏大学制药工程系江苏镇江,江苏镇江,江苏镇江

来源：

药学学报 | 2002年 / 07期

关键词：

银杏酸; 银杏叶; LC/MS; 反相高效液相色谱法;

D O I：

10.16438/j.0513-4870.2002.07.013

中图分类号：

O657.7 [色谱分析];

学科分类号：

摘要：

目的　建立银杏酸的简便预净化处理和HPLC含量测定方法。方法　用LC DAD ESI MS对银杏酸进行定性鉴定。银杏叶经正己烷提取、硅胶柱色谱净化处理 ,HPLC测定。色谱分析条件 :色谱柱为InertsilODS 2 ,流动相为甲醇 3%HAc溶液 (92∶8) ,流速 1 0mL·min- 1 ,柱温 40℃ ,紫外检测波长 310nm。结果　银杏叶中存在 5种银杏酸C13∶0 ,C15∶0 ,C15∶1,C17∶1和C17∶2 ,其中C15∶1和C17∶1约占银杏酸的 85 % ,C17∶2银杏酸未见国内文献报道。银杏叶提取物经硅胶柱色谱处理后 ,其HPLC谱中除银杏酸峰外 ,几无其他杂质峰。平均回收率 97 0 % ,RSD为 1 7%(n =6 )。结论　该方法准确、简便、可靠 ,可用于银杏酸的定量分析。

引用

页码：555 / 558

页数：4

共 7 条

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