铁蓄积对骨内H型血管影响的实验研究

被引：5

作者：

王亮 ^{[1
]}

韩小娟 ^{[1
]}

赵国阳 ^{[2
]}

单宇 ^{[3
]}

王爱飞 ^{[1
]}

刘志鹏 ^{[1
]}

陈斌 ^{[1
]}

俞晨 ^{[1
]}

徐又佳 ^{[1
]}

机构：

[1] 苏州大学附属第二医院骨科

[2] 镇江, 江苏大学附属医院骨科

[3] 苏州, 吴江区第一人民医院骨科

来源：

中华骨科杂志 | 2017年 / 37卷 / 14期

关键词：

动物实验; 骨密度; 骨质疏松;

D O I：

暂无

中图分类号：

R580 [];

学科分类号：

摘要：

目的探讨铁蓄积导致骨量下降的过程中骨内H型血管的变化和相关意义。方法选取10只雄性8周龄野生型C57BL/6J小鼠, 随机分为对照组及铁蓄积组, 每组5只。对铁蓄积组腹腔注射0.1g/kg右旋糖酐铁, 1次/周, 连续注射8周, 对照组注射等量生理盐水。取股骨、胫骨标本分别进行骨组织Micro-CT扫描、骨组织H型血管免疫荧光染色。收集两组小鼠肝脏组织通过原子吸收法检测铁含量变化, 并对各组间的结果进行统计学分析。结果铁蓄积小鼠肝铁含量较对照组显著升高, 模型制作成功。收集两组小鼠胫骨进行免疫荧光实验:铁蓄积组小鼠胫骨干骺端骨内H型血管面积为11.24%±1.76%, 对照组H型血管面积为30.69%±2.78%, 两组间血管面积差异有统计学意义(P< 0.005)。收集两组小鼠股骨行Micro-CT扫描:铁蓄积组骨密度(bone mineral density, BMD), 体积分数(bone volume/tissue volume, BV/TV), 骨小梁厚度(trabecular thickness, Tb.Th), 骨小梁数目(trabecular number, Tb.N), 骨小梁间隙(trabecular separation, Tb.Sp)分别为(0.19±0.013)g/cm3, 11.92%±1.199%, (35.66±2.684)μm, (2.36±0.429)个/mm, (284.41±23.197)μm;对照组BMD, BV/TV, Tb.Th, Tb.N, Tb.Sp为(0.37±0.023)g/cm3, 35.76%±1.336%, (62.05±2.238)μm, (5.68±1.039)个/mm, (163.23±13.203)μm, 两组间差异均有统计学意义(P< 0.05)。结论铁蓄积导致了骨密度下降, 同时骨内H型血管的形成显著受到抑制, 这对了解铁蓄积导致骨质疏松症的作用机理有新的实验价值。

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