普罗布考对小鼠体内巨噬细胞胆固醇逆转运的影响及其作用机制

被引：5

作者：

王丽霞

黄彦生

罗萍

倪占玲

段红艳

杨海涛

许先静

机构：

[1] 河南省人民医院老年临床医学部心内科

来源：

中华老年心脑血管病杂志 | 2013年 / 15卷 / 01期

关键词：

普罗布可; 巨噬细胞; 胆固醇; 动脉粥样硬化; 降血脂药; 干预性研究;

D O I：

暂无

中图分类号：

R543.5 [动脉疾病];

学科分类号：

1002 ; 100201 ;

摘要：

目的探讨普罗布考对小鼠体内巨噬细胞TC逆转运的影响及其作用机制。方法选择C57BL/6小鼠36只随机分为4组,给予不同剂量普罗布考(0,0.1%,0.5%,1.0%,分别为对照组,0.1%干预组,0.5%干预组,1.0%干预组),各组9只。添加饲料喂养6周后,腹腔注射乙酰化LDL与氚(3 H)-TC标记的RAW264.7小鼠巨噬细胞悬液,酶法测定血脂,用液闪计数仪测定血清、肝脏及粪便中3 H-TC占经腹腔注射3 H-TC的百分比。结果普罗布考干预6周后,与对照组比较,各干预组小鼠TC、HDL-C、LDL-C明显降低(P<0.05,P<0.01),肝脏和粪便中3 H-TC含量明显升高(P<0.05),血清3 H-TC含量明显降低(P<0.05)。各干预组HDL-C水平与3 H-TC排泄量呈负相关(r=-0.532,P<0.05)。结论普罗布考干预了小鼠体内巨噬细胞TC逆转运,加速了TC经粪便的清除,有利于动脉粥样硬化的防治。

引用

页码：74 / 76

页数：3

共 12 条

[1]

Frequency and healing of noncul-prit coronary artery plaque disruptions in patients with acute myoc-ardial infarction. Okada K,Ueda Y,Matsuo K,et al. The American Journal of Cardiology . 2011

[2] Overexpression of Human 15(S)-Lipoxygenase-1 in RAW Macrophages Leads to Increased Cholesterol Mobilization and Reverse Cholesterol Transport [J].

Weibel, Ginny L. ;

Joshi, Michelle R. ;

Alexander, Eric T. ;

Zhu, Peijuan ;

Blair, Ian A. ;

Rothblat, George H. .

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2009, 29 (06) :837-U159

[3]

Dietary cholesterol affects plasmalipid levels,the intravascular processing of lipoproteins an-dReverse cholesterol transport without increasing the risk forheart disease. Barona J,Fernandez ML. Nutrients . 2012

[4]

Role of matrixmetalloproteinase-2in the cardioprotective effect of ischaemicpostconditioning. Donato M,Annunzio V,Buchholz B,et al. Experimental Physiology . 2010

[5]

Matrix metalloproteinase-2and myocardial oxidativestress injury: beyond the matrix. Kandasamy AD,Chow AK,Ali MA. Cardiovascular Research . 2010

[6]

Chymase inhibitionreduces infarction and matrix metalloproteinase-9activation andattenuates inflammation and fibrosis after acute myocardialischemia/reperfusion. Oyamada S,Bianchi C,Takai S,et al. Journal of Pharmacology and Experimental Therapeutics . 2011

[7]

Antiatherogeniceffects of cilostazol and probucol alone,and in combination inlow density lipoprotein receptor-deficient mice fed with a highfat diet. Yoshikawa T,Mitani K,Kotosai K,et al. Hormone and Metabolic Research . 2008

[8]

Antioxidant probucol at-tenuates myocardial oxidative stress and collagen expressionsin post-myocardial infarction rats. Zhou SX,Zhou Y,Zhang YL,et al. Journal of CardiovascularPharmacology . 2009

[9]

The LXR agonist T0901317 promotes the reverse cholesterol transport from macrophages by increasing plasma efflux potential. Zanotti I,Poti F,Pedrelli M,Favari E, et al. Journal of Lipid Research . 2008

[10]

Advanced glycated al-bumin impairs HDL anti-inflammatory activity and primes macrophages for inflammatory response that reduces reverse cholesterol transport. Okuda LS,Castilho G,Rocco DD,et al. Biochimica et Biophysica Acta . 2012

← 1 2 →