一株兼性嗜冷小甲烷粒菌的生物学特性及系统发育分析

被引:4
作者
袁敏 [1 ,2 ]
张辉 [1 ,2 ]
胡国全 [1 ,2 ]
机构
[1] 不详
[2] 农业部沼气科学研究所
[3] 不详
[4] 农业部能源微生物与利用重点开放实验室
[5] 不详
关键词
小甲烷粒菌; 产甲烷菌; 16S rDNA; 系统发育分析;
D O I
暂无
中图分类号
X172 [环境微生物学];
学科分类号
摘要
采用改进的亨盖特(Hungate)厌氧技术,从西藏林芝厌氧消化系统中分离到一株产甲烷菌菌株LZ-6.该菌株为革兰氏阴性,不运动,球形,直径约0.3~0.6μm.该菌株利用H2/CO2,微利用甲酸生长,不利用乙酸、甲醇、甲醇/H2、三甲胺、甲胺,最适生长pH为6.8~7.2,最适生长温度25℃,最适Na+浓度0.2mol/L.菌株LZ-6的16SrRNA基因序列与小甲烷粒菌(Methanocorpusculum parvum)相似性为99%.生理、形态结构特征等生物学特性研究表明此株产甲烷古菌为兼性嗜冷产甲烷古菌.
引用
收藏
页码:705 / 709
页数:5
相关论文
共 5 条
[1]   Isolation and characterization of new strains of methanogens from cold terrestrial habitats [J].
Simankova, MV ;
Kotsyurbenko, OR ;
Lueders, T ;
Nozhevnikova, AN ;
Wagner, B ;
Conrad, R ;
Friedrich, MW .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 2003, 26 (02) :312-318
[2]  
Methanogenium marinum sp. nov., a H2-using methanogen from Skan Bay, Alaska, and kinetics of H2 utilization[J] . Song C. Chong,Yitai Liu,Michael Cummins,David L. Valentine,David R. Boone.Antonie van Leeuwenhoek . 2002 (1)
[3]  
Methanosarcina lacustris sp. nov., a New Psychrotolerant Methanogenic Archaeon from Anoxic Lake Sediments[J] . Maria V. Simankova,Sofja N. Parshina,Tatjana P. Tourova,Tatjana V. Kolganova,Alexander J.B. Zehnder,Alla N. Nozhevnikova.Systematic and Applied Microbiology . 2001 (3)
[4]  
Methanocorpusculaceae fam. nov., represented by Methanocorpusculum parvum, Methanocorpusculum sinense spec. nov. and Methanocorpusculum bavaricum spec. nov[J] . G. Zellner,E. Stackebrandt,P. Messner,B. J. Tindall,E. Conway de Macario,H. Kneifel,U. B. Sleytr,J. Winter.Archives of Microbiology . 1989 (5)
[5]  
Isolation and characterization of Methanocorpusculum parvum , gen. nov., spec. nov., a new tungsten requiring, coccoid methanogen[J] . G. Zellner,C. Alten,E. Stackebrandt,E. Conway de Macario,J. Winter.Archives of Microbiology . 1987 (1)