同型半胱氨酸对大鼠血管平滑肌细胞分泌和表达IL-6的影响

被引：11

作者：

朱建华

张力

机构：

[1] 浙江大学医学院附属第一医院心内科,浙江大学医学院附属第一医院心内科浙江杭州,浙江杭州

来源：

中国病理生理杂志 | 2002年 / 09期

关键词：

高半胱氨酸; 白细胞介素6; 动脉粥样硬化;

D O I：

暂无

中图分类号：

R363 [病理生理学];

学科分类号：

摘要：

目的 :探讨同型半胱氨酸 (Hcy )对大鼠血管平滑肌细胞 (VSMCs)分泌和表达促炎性细胞因子白细胞介素 - 6 (IL - 6 )的影响。方法 :在大鼠主动脉平滑肌细胞培养基中加入不同浓度的Hcy ,并孵育不同时间。用ELISA法检测培养上清IL - 6蛋白含量 ,用半定量RT -PCR法检测IL - 6mRNA表达。结果 :0 2 5mmol/LHcy干预后 ,IL - 6在 6h开始高于对照组 ,2 4h达峰值 ,4 8h仍较对照组高 ;并且在一定的浓度范围内 ,Hcy呈剂量依赖性刺激VSMCs产生IL - 6 ,0 1mmol/L及 0 2 5mmol/LHcy分别使IL - 6产量较对照增多 1 4和 3 4倍。RT -PCR结果显示Hcy能刺激IL - 6mRNA表达 ,也呈剂量和时间依赖关系。结论 :Hcy能促进VSMCs产生和表达IL - 6、激活血管壁炎症反应 ,这可能为其诱导动脉粥样硬化的重要机制之一。

引用

页码：103 / 106

页数：4

共 9 条

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