根癌农杆菌介导AtNHX1基因转化小麦

被引：17

作者：

薛哲勇

支大英

夏光敏

马秀玲

张慧

赵彦修

机构：

[1] 山东大学生命科学学院

[2] 山东师范大学植物逆境实验室

[3] 山东师范大学植物逆境实验室济南

[4] 济南

来源：

山东大学学报(理学版) | 2003年 / 01期

关键词：

小麦; 根癌农杆菌; 转基因植株; AtNHX1基因;

D O I：

暂无

中图分类号：

Q943 [植物细胞遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

利用根癌农杆菌对 3种基因型小麦进行了转化研究 .发现基因型对转化有明显影响 .将小麦品种烟优 36 1、烟 10 3、核生 3号的胚性愈伤组织用含报告基因NPTⅡ和抗盐基因Na+ H+反向转运AtNHX1的农杆菌菌株pROK2AT GV310 1感染和共培养后 ,用巴龙霉素 5 0 - 15 0mg L筛选抗性愈伤组织及转化植株 .对抗性植株的总DNA用AtNHX1基因的特异引物进行PCR检测 ,目的基因的PCR阳性频率为 1.3(烟 10 3)和 2 .9% (核生 3号 ) .Southern杂交证实外源基因已经整合到小麦的基因组中

引用

页码：106 / 109

页数：4

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