乳酸菌发酵产生共轭亚油酸条件的研究

被引：26

作者：

邵群

边际

马丽

张慧

机构：

[1] 山东师范大学生物学系

[2] 国家海洋局第一研究所

[3] 山东师范大学化学系

来源：

山东师大学报(自然科学版) | 2001年 / 04期

关键词：

嗜酸乳酸杆菌1.1854; 吸收; 共轭亚油酸;

D O I：

暂无

中图分类号：

Q936 [微生物生物化学];

学科分类号：

071010 ;

摘要：

分别用嗜酸乳酸杆菌Lactobacillusacidophilus 1.185 4、干酪乳杆菌 (L .casei)Bs5、Bs7及乳酸乳球菌(Lactococcuslactis)L318进行发酵 ,培养液中添加一定量的亚油酸 ,发酵物用正己烷萃取纯化 ,测定其紫外吸收发现 ,菌株 1.185 4、Bs5、Bs7均在 2 34nm处得到共轭亚油酸的特征吸收峰 ,其中菌株 1.185 4的吸收值最大 .在MRS培养基中加入 0 .1%亚油酸 ,接种菌株 1.185 4,37℃静置培养 2 4h ,为产生共轭亚油酸的最佳条件 .用脱脂牛奶作培养基 ,获得了与上述相同的结果 .以纯品共轭亚油酸为标准 ,在 2 34nm处作标准吸收曲线 ,可计算出发酵物中共轭亚油酸的含量

引用

页码：443 / 446

页数：4

共 9 条

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