宣白承气汤对肠源性脓毒症大鼠保护的信号转导机制研究

被引：7

作者：

孟繁甦

郭应军

侯杰

刘八一

王国军

陈华琼

李平

林冰

机构：

[1] 中山市中医院ICU

来源：

四川中医 | 2011年 / 29卷 / 03期

基金：

广东省自然科学基金;

关键词：

宣白承气汤; 肠源性脓毒症; 信号转导通路;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理];

学科分类号：

1008 ;

摘要：

目的:探讨宣白承气汤对脓毒症大鼠保护作用的细胞信号转导机制。方法:SD大鼠30只,随机分为3组,模型组、假手术组和治疗组各10只。实验期间大鼠禁食不禁水。模型组和治疗组采用盲肠结扎穿刺法,假手术组仅翻动盲肠,不做穿刺结扎处理。治疗组在CLP后8h、12h和24h时宣白承气汤剂按1ml/100g体质量灌胃。第48h时(即实验结束时)心脏取血3ml离心后去上清液测定TNFα、IL-6含量。并取距小肠回盲部上端15cm处的肠管2cm,称重后按每1g湿重加入4℃的PBS10ml比例,冰浴匀浆约1min后,4℃放置4500转/min离心30min后取上清备用。免疫印迹法检测小肠组织中p-p38、total-p38丝裂原活化蛋白激酶表达水平。结果:模型组的TNFα及IL-6浓度与假手术组比较,均明显升高(P<0.05);与模型组比较,治疗组可明显降低血清中TNFα及IL-6浓度(P<0.05)。模型组p-p38/p-38灰度比值为0.48±0.09,假手术组的灰度比为0.16±0.03,治疗组的灰度比为0.21±0.05。与假手术组比较,模型组灰度比值明显增加(P<0.05);与模型组比较,治疗组的灰度比值明显降低(P<0.05)。结论:宣白承气汤保护肠源性脓毒症大鼠的作用机制与抑制小肠组织中p38丝裂原活化蛋白激酶信号通路有关,进而减少炎症介质TNFα、IL-6释放。

引用

页码：31 / 32

页数：2

共 8 条

[1]

Role of the gut in multiple organ failure: bacterial translocation and permeability changes. Swank GM,Deitch EA. World Journal of Surgery . 1996

[2]

Selec-tive inhibition of TNF-alpha or IL-1 beta does not affect E.Coli-in-duced inflammation in human whole blood. Barratt-Due A,Thorgersen EB,Lindstad JKet al. Molecular Immunology . 2010

[3]

Sepsis-induced muscle proteolysis is prevented by a proteasome inhibitor in vivo. Fischer D,Gang G,Pritts T,et al. Biochemical and Biophysical Research Communications . 2000

[4]

Treatment of septic shock with the tumor necrosis factor receptor: Fc fusion protein. Fisher C H J,Agosti J M,Opal S M,et al. The New England Journal of Medicine . 1996

[5]

Madecassoside suppressesLPS-induced TNF-alpha production in cardiomyocytes throgh inhibition ofERK p38 and NF-kappaB activity. Cao W,Li XQ,Zhang XNet al. INt Innmunopharmacol . 2010

[6]

Increase in proinflammatory cytokines in peripheral blood without haemostatic changes after LPS inhalation. Fouassier M,Souweine B,Sapin AFet al. Thrombosis Research . 2009

[7]

The gut: a central organ after surgical stress. Wilmore DW,Smith RJ,O’Dwyer ST,et al. Journal of Surgery . 1988

[8]

Lipopolysaccharide activates distinct signaling pathways in intestinal epithelial cell lines expressing Toll-like receptors. Cario E,Rosenberg IM,Brandwein SL,et al. Journal of Immunology . 2000

← 1 →