BDNF、NGF对体外培养的胚胆碱能神经元生长发育的影响

被引:9
作者
金国华
徐慧君
武义鸣
田美玲
钟世镇
机构
[1] 南通医学院神经生物学研究室!南通
[2] 不详
[3] 第一军医大学解剖学教研室!广州
关键词
BDNF; NGF; 胆碱能神经元; AChE; 生长发育; 细胞培养; 鼠胚;
D O I
暂无
中图分类号
R33 [人体生理学];
学科分类号
摘要
本文用AChE组化方法,研究了BDNF、NGF对培养的胚鼠基底前脑胆碱能神经元的作用及BDNF和NGF的协同作用。结果表明BDNF和NGF都具有增加AChE阳性神经元数量的作用,二者的不同在于BDNF作用出现的时间较早、强度较小;而NGF作用出现的时间较迟但强度较大。并发现BDNF对体外培养的胚胆碱能神经元胞体早期的生长发育作用比较明显,而NGF的作用则不甚显著。BDNF对胚胆碱能神经元发出突起和突起的延伸作用较NGF强。BDNF和NGF的联合作用较单独使用BDNF或NGF为好。本文的结果提示在体外培养中两种营养因子联合应用较只用一种因子有益。
引用
收藏
页码:230 / 234
页数:5
相关论文
共 14 条
[1]  
Dealopmental change in the nerve growth factor action from induction ofcholine acetyltransferase to promotion of cell survival in cultured basal forebrain cholinergic neurons from postnatalrats. Hatanaka H,Tsukui H,Nihonmatsu I. Developmental Brain Research . 1988
[2]  
Neuron[P]. KUTKOVETSKYI VALENTYN YAKOVYCH;TURTI MARYNA VALENTYNIVNA.UA106289U,2016-04-25
[3]  
Neuron[P]. KUTKOVETSKYI VALENTYN YAKOVYCH;TURTI MARYNA VALENTYNIVNA.UA106289U,2016-04-25
[4]  
The trk proto-oncogene encodes a receptor for nerve growth factor. Klein R,Jing SQ,Nanduri V,et al. Cell . 1991
[5]  
Promotion of central cholinergic and dopaminergic neuron differentiationby brain-derived neurotrophic factor but not neurotrophin 3. Knusel B,Winslow JW,Rosenthal A,et al. Proceedings of the National Academy of Sciences of the United States of America . 1991
[6]  
The neurotrophic lactors bram-drived neurolrophie factor and neurotrophin3 are ligands for the trkB tyrosine kinase receptor. Soppet D,Escandon E,Mariagos J. et al. Cell . 1991
[7]  
Neuron[P]. KUTKOVETSKYI VALENTYN YAKOVYCH;TURTI MARYNA VALENTYNIVNA.UA106289U,2016-04-25
[8]  
Molecular cloning and neurotrophic activities of a protein with structural simi-larities to nerve growth factor: developmental and topographical expression in the brain. Ernfors P,Ibanez CF,Ebendal T,et al. Proceedings of the National Academy of Sciences of the United States of America . 1990
[9]  
Visualization of detailed acetylcholinesterase fiber and neuron staining in rat brain by asensitive bistochemical procedure. Tago H,Kimura H,Maeda T. Journal of Histochemistry and Cytochemistry . 1986
[10]  
Neuron[P]. KUTKOVETSKYI VALENTYN YAKOVYCH;TURTI MARYNA VALENTYNIVNA.UA106289U,2016-04-25