有机磷类杀虫剂对脑突触体烟碱型自主受体功能的影响

被引：4

作者：

伍一军

孙英健

鲍凯瑞

机构：

[1] 中国科学院动物研究所分子毒理学实验室,北京农学院动物医学系,美国俄克拉何马州立大学动物医学院生理科学系北京

来源：

中华劳动卫生职业病杂志 | 2003年 / 03期

基金：

中国科学院知识创新工程重大项目;

关键词：

杀虫药,有机磷; 受体,烟碱; 乙酰胆碱酯酶;

D O I：

暂无

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

目的　探讨有机磷杀虫剂 (OPs)对脑突触体烟碱型自主受体功能 (NAF)的影响 ,揭示OPs可能存在的其他作用途径。方法　 (1)体外实验 :以放射性核素氚标记的胆碱与大鼠脑突触体共同温育后用生理缓冲液灌流 ,根据不同有机磷刺激时的放射性胆碱释放量计算NAF值 ,分析氧毒死蜱和对氧磷分别加入灌流系统后NAF的变化 ;(2 )体内实验 :用毒死蜱给大鼠染毒 ,96h后取其大脑皮质制备突触体 ,测定其NAF和乙酰胆碱酯酶 (AChE)活力。结果　对氧磷在体外对NAF影响较小(平均抑制率 <2 3% ) ,但氧毒死蜱却彻底抑制NAF(平均抑制率 >10 0 % )。体内实验亦显示 ,毒死蜱在抑制AChE活力 (抑制率为 91% )的同时 ,对NAF有较强的阻断作用 (抑制率为 6 6 % )。结论　不同的OPs对脑突触体NAF的作用不同 ,毒死蜱可阻断成年大鼠脑突触体的NAF。

引用

页码：31 / 33

页数：3

共 9 条

[1]

Glucose feeding exacerbates parathion-induced neurotoxicity. Olivier K,Liu J,Karanth S,et al. Journal of Toxicology and Environmental Health . 2001

[2]

A rapid, simple radiometric assay for cholinesterase, suitable for multiple determinations. Johnson CD,Russell RL. Analytical Biochemistry . 1975

[3]

Age-related differences in parathion and chlorpyrifos toxicity in male rats: target and nontarget esterase sensitivity and cytochrome P450-mediated metabolism. Atterberry TT,Burnett WT,Chambers JE. Toxicology and Applied Pharmacology . 1997

[4]

Age-related effects of chlorpyrifos on acetylcholine release in rat brain. Won YK,Liu J,Olivier K Jr,et al. Neurotoxicology . 2001

[5]

Organophosphorus pesticides: do they all have the same mechanism of toxicity. Pope CN. Journal of Toxicology and Environmental Health . 1999

[6]

Properties of neuronal nicotinic acetylcholine receptors: pharmacological characterization and modulation of synaptic function. Albuquerque EX,Alkondon M,Pereira EF,et al. Journal of Pharmacology and Experimental Therapeutics . 1997

[7]

Carboxylesterase and A-esterase activities during maturation and aging: relationship to the toxicity of chlorpyrifos and parathion in rats. Karanth S,Pope C. Toxicological Sciences . 2000

[8]

Heterogeneity of presynaptic muscarinic receptors regulating neurotransmitter release in the rat brain. Raiteri M,Leardi R,Marchi M. Journal of Pharmacology and Experimental Therapeutics . 1984

[9]

Nicotinic receptors modulating ACh release in rat cortical synaptosomes: role of Ca2+ ions in their function and desensitization. Marchi M,Lupinacci M,Bernero E,et al. Neurochemistry International . 1999

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