吲哚美辛诱导肝癌细胞凋亡及与环氧合酶-2和凋亡调控基因表达关系

被引：4

作者：

谢勇

吕农华

周小江

黄德强

陈江

王崇文

机构：

[1] 江西医学院第一附属医院消化研究所

[2] 江西医学院第一附属医院消化研究所南昌

[3] 南昌

来源：

中华消化杂志 | 2004年 / 10期

关键词：

吲哚美辛; 环氧合酶-2; 肝细胞癌; 凋亡;

D O I：

暂无

中图分类号：

R735.7 [肝肿瘤];

学科分类号：

100214 ;

摘要：

目的探讨非甾体类抗炎药(MSAIDs)诱导肝癌细胞株SMMC7721细胞凋亡的作用及可能机制,为其应用于肝细胞癌的化学预防和治疗提供实验和理论依据。方法采用TUNEL法和免疫细胞化学方法研究了不同浓度吲哚美辛对肝癌细胞株SMMC7712的诱导凋亡作用,及其对环氧合酶(COX)2和凋亡相关基因p53、p27kipl、Cmyc蛋白和Fas抗原表达的影响。结果①对照组、100、200、400、600和800μmol/L吲哚美辛组肝癌细胞的细胞凋亡指数分别为(2.4±1.4)%、(4.2±1.5)%、(8.0±3.2)%、(14.3±5.1)%、(14.9±4.3)%和(19.0±6.1)%(P<0.0001)。②对照组、100、200、400、600和800μmol/L吲哚美辛组COX2的表达强度分别为强阳性、强阳性、强阳性、阳性、弱阳性和弱阳性。③对照组、100、200、400、600和800μmol/L吲哚美辛组p27kipl的阳性积分分别为0,3.7±1.4,12.3±2.4,26.3±4.5,44.4±5.1和60.6±7.8(P<0.0001);p53阳性积分分别为5.3±1.2,6.1±2.3,24.1±6.1,46.2±6.9,47.3±6.6和49.0±5.1(P<0.0001);Cmyc的阳性积分分别为5.8±1.6,5.9±1.7,18.6±6.3,22.0±5.6,44.0±8.4,和44.3±6.1(P<0.0001);Fas阳性积分分别为21.7±6.0,24.2±5.5,25.7±5.9,24.3±5.4,23.1±6.6和22.3±4.3(P>0.05)。结论吲哚美辛可诱导肝癌细胞凋亡,且与其

引用

页码：31 / 34

页数：4

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