慢性脑低灌注大鼠海马神经元损伤的机制研究

被引：9

作者：

刘晖

章军建

杨英

张磊

机构：

[1] 武汉大学中南医院神经内科

来源：

中华行为医学与脑科学杂志 | 2011年 / 01期

关键词：

慢性脑低灌注; 大鼠模型; 海马神经元; 细胞损伤; 凋亡;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理];

学科分类号：

100806 [中药药理学];

摘要：

目的探究慢性脑低灌注大鼠海马神经元损伤的机制。方法双侧颈总动脉结扎(2VO)制备慢性脑低灌注大鼠模型,2VO术后8周取材,分别进行HE染色、电镜、流式细胞术以及Western Blotting检测。结果 HE结果显示2VO组的海马神经元排列稍紊乱,且数量与假手术对照相比有不同程度减少[CA2区:(34.75±3.40)个,(49.25±9.67)个,P<0.05;DG区:(73.50±9.26)个,(90.75±4.35)个,P<0.05];电镜观察发现2VO组大鼠海马区部分神经元胞核中略有固缩,出现异染色质边集,胞浆则出现水肿,以线粒体与内质网尤甚;流式细胞术结果表明2VO组海马神经元早期凋亡率[(9.117±2.540)%]高于假手术对照组[(4.750±3.481)%],差异有统计学意义(P<0.05);WesternBlotting检测发现procaspase-3在2VO组大鼠海马的表达与假手术对照组差异无显著性(P>0.05)。结论慢性脑低灌注大鼠海马神经元的损伤主要由凋亡造成。

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