海南长春花黄化病植原体的16S rDNA序列分析研究

被引：17

作者：

车海彦 ^{[1
,2
]}

罗大全 ^{[2
]}

符瑞益 ^{[2
]}

叶莎冰 ^{[2
]}

吴云锋 ^{[1
]}

机构：

[1] 西北农林科技大学植物保护学院陕西省农业分子生物学重点实验室

[2] 中国热带农业科学院环境与植物保护研究所

来源：

植物病理学报 | 2009年 / 02期

关键词：

D O I：

暂无

中图分类号：

S436.8 [观赏园艺类病虫害];

学科分类号：

090401 [植物病理学];

摘要：

Periwinkle(Catharanthus roseus) yellows is a common disease in Hainan. Periwinkle′s leaf tissue with symptoms was assayed for phytoplasma infection by using PCR assay employing phytoplasma universal 16S rRNA gene primers (Rl6mF2/Rl6mR1). A PCR product (about 1.4 kb) was amplified from periwinkle showed yellows. Nucleotide sequencing and phylogenetic tree analysis showed that the amplified 16S rDNA contained 1 432 nucleotides, the most homology was 98.1% with the members of elm yellows group (16S r Ⅴ) and clustered in the same clade, while it was under 96.1% with other phytoplasma groups. Our results suggested that the phytoplasma sample belonged to 16S rⅤgroup and was tentatively named as Hainan periwinkle yellows phytoplasma (PY-Hn). This is the first report of existence of 16S r Ⅴ group phytoplasma in naturally infected periwinkle.

引用

页码：212 / 216

页数：5