氟哌啶醇对大鼠离体海马脑片和原代神经元缺糖/缺氧和N-甲基-D-天冬氨酸损伤的保护作用

被引：4

作者：

严乐勤

魏尔清

沈建中

沈波

机构：

[1] 浙江大学医学院神经生物学实验室、药理学实验室

[2] 浙江大学医学院神经生物学实验室、药理学实验室浙江杭州

[3] 浙江杭州

来源：

药学学报 | 2002年 / 12期

基金：

浙江省自然科学基金;

关键词：

氟哌啶醇; 海马; 原代神经元; N-甲基-D-天冬氨酸;

D O I：

10.16438/j.0513-4870.2002.12.003

中图分类号：

R965 [实验药理学];

学科分类号：

100602 ; 100706 ;

摘要：

目的　观察氟哌啶醇对大鼠离体海马脑片和原代神经元的缺糖缺氧 (OGD)和N 甲基 D 天冬氨酸 (N methyl D aspartate ,NMDA)损伤的潜在保护作用及其机制。方法　海马脑片OGD以无葡萄糖的人工脑脊液中通 95 %N2 +5 %CO2 诱导。通过测定TTC染色后形成的红色产物来分析脑片活性。结果　氟哌啶醇 (1和 10 μmol·L- 1 )抑制OGD损伤 ,抑制率分别为 17 7%和 2 5 %,而D2 多巴胺受体拮抗剂多潘立酮无此作用。NMDA也能显著降低海马脑片及原代神经元的活性 ,而氟哌啶醇可抑制这一损伤作用。结论　氟哌啶醇对大鼠离体海马脑片OGD和原代神经元NMDA损伤有保护作用。

引用

页码：922 / 926

页数：5

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