冬性花椰菜的小孢子胚诱导和植株再生研究

被引：26

作者：

顾宏辉

唐桂香

张国庆

周伟军

机构：

[1] 浙江大学农学系

[2] 浙江大学农学系浙江杭州浙江省农业科学院作物研究所浙江杭州

[3] 浙江杭州

来源：

浙江大学学报(农业与生命科学版) | 2004年 / 01期

基金：

教育部留学回国人员科研启动基金;

关键词：

花椰菜(Brassicaoleraceavar.botrytis); 小孢子培养; 胚胎发生; 植株再生; 倍性检测;

D O I：

暂无

中图分类号：

S635.3 [花椰菜];

学科分类号：

090202 ;

摘要：

以2个冬性花椰菜F1杂交种为供体材料,研究了不同热击温度与时间组合、更新培养液和冷击预处理对花椰菜小孢子培养胚发频率的影响.结果表明,最佳热击组合是32℃、24h;先用17%蔗糖浓度的NLN-17培养液热击培养24h后换成10%蔗糖的NLN-10培养液,并转入24℃继续培养,能显著提高出胚产量;更换培养液和冷击预处理能明显提高胚体质量,减少愈伤状胚的形成.分离小孢子在NLN-13培养液中经32℃、24h热击暗培养后转入24℃下培养,产生了大量正常胚体.小孢子分离30d后将正常胚体移入固体MS培养基中萌发、长根,并经2次继代培养后获得了大量正常的再生植株,其多为自发双单倍体.

引用

页码：37 / 41

页数：5

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