脑缺血再灌注后脑组织白介素-1β转化酶基因的表达

被引:1
作者
张金涛
吴卫平
王树新
刘洁晓
张风英
机构
[1] 解放军总医院神经内科
关键词
脑缺血; 再灌注损伤; 凋亡; 白介素-1β转化酶; 基因表达;
D O I
10.14172/j.cnki.issn1671-4008.2000.03.005
中图分类号
R364 [病理过程];
学科分类号
摘要
为探讨白介素 -1 β转化酶mRNA在前脑缺血再灌注后 1~ 4d被诱导表达及定位的情况 ,应用逆转录PCR方法检测沙土鼠前脑缺血再灌注模型白介素 -1 β转化酶mRNA的诱导情况 ;同时应用免疫组化方法对白介素 -1 β转化酶定位表达进行观察。结果白介素 -1 β转化酶mRNA在脑缺血再灌注后的 1~ 4d被诱导表达 ;非缺血海马区及皮层仅见锥体细胞及中间神经元白介素 -1 β转化酶的轻度表达 ;而白介素 -1 β转化酶在胶质细胞的表达在缺血后的 1~ 4d均可观察到 ,且逐渐增加 ,以第 3d最明显。结果证实了白介素 -1 β转化酶基因在脑缺血再灌注后的表达和定位 ,说明白介素 -1 β转化酶可能参与脑组织因缺血而发生的凋亡的发生过程。
引用
收藏
页码:136 / 138
页数:3
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