TGF-β1参与四氯化碳诱导小鼠急性肝损伤

被引：4

作者：

牛立慢

齐妍

吴倩

崔雪玲

柳忠辉

葛敬岩

机构：

[1] 吉林大学基础医学院免疫学系

来源：

中国公共卫生 | 2016年 / 06期

关键词：

四氯化碳; 急性肝损伤; 转化生长因子β1; Smad2; Smad3;

D O I：

暂无

中图分类号：

R575 [肝及胆疾病];

学科分类号：

100201 [内科学];

摘要：

目的探讨转化生长因子β1(TGF-β1)是否参与四氯化碳(CCl4)诱导的小鼠急性化学性肝损伤过程。方法采用腹腔注射CCl4诱导小鼠急性肝损伤,ELISA法检测血清TGF-β1水平,RT-PCR检测TGF-β信号分子Smad2、3的表达。结果与橄榄油对照小鼠比较,CCl4处理1d及3d的急性肝损伤小鼠不仅血清转氨酶ALT与AST水平升高,血清TGF-β1水平也明显升高,差异有统计学意义(P<0.01);RT-PCR结果显示,CCl4处理小鼠肝组织TGF-β1 mRNA表达水平明显高于橄榄油对照小鼠,其受体TβRII及信号蛋白Smad2与Smad3 mRNA也表达升高。结论 CCl4诱导小鼠急性肝损伤时TGF-β1及其信号分子Smad2、3表达增加,提示TGF-β1参与CCl4诱导小鼠肝损伤过程。

引用

页码：795 / 798

页数：4

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