类风湿性关节炎患者血清和滑液IL-18及IL-18结合蛋白的水平及意义

被引:9
作者
王叶萍
姚航平
机构
[1] 浙江省长兴县人民医院检验科
[2] 浙江大学医学院传染病研究所 浙江长兴
[3] 浙江杭州
基金
浙江省自然科学基金;
关键词
白细胞介素-18; 白细胞介素-18结合蛋白; 类风湿性关节炎;
D O I
暂无
中图分类号
R593.22 [类风湿性关节炎];
学科分类号
1002 ; 100201 ;
摘要
目的 研究类风湿性关节炎 (RA)患者血清、滑液中白细胞介素 18(IL 18)及IL 18结合蛋白 (IL 18BP)的水平 ,探讨他们在RA致病中的作用。方法 应用双抗体夹心酶联免疫吸附试验 (ELISA)和生物学方法分别测定RA患者血清、滑液中IL 18及IL 18BP水平和生物活性 ,同时采用硝酸酶还原法及竞争ELISA分别检测一氧化氮 (NO)和前列腺素E2 (PGE2 )的含量。以因外伤截肢者作对照。结果 RA患者血清、滑液中IL 18水平和生物活性均显著高于对照组 ,且滑液中量及活性比血清中高 ;而IL 18BP水平RA患者明显低于对照组。结论 IL 18的过度表达以及IL 18BP表达的相对不足 ,可能是RA发病的机制之一 ;选择抑制过度表达的IL 18生物活性 ,及增加IL 18BP的表达 ,将是RA治疗的新途径。
引用
收藏
页码:518 / 520
页数:3
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