多重PCR对金黄色葡萄球菌杀白细胞素基因的检测

被引：26

作者：

余方友

陈增强

林晓梅

李美兰

张雪青

陈占国

陈坚

周铁丽

机构：

[1] 温州医学院附属第一医院检验科

来源：

中华微生物学和免疫学杂志 | 2006年 / 12期

关键词：

金黄色葡萄球菌; Panton-Valentine杀白细胞素基因; SCCmec; 多重PCR;

D O I：

暂无

中图分类号：

R450 [];

学科分类号：

100215 ;

摘要：

目的应用多重PCR检测含Panton-Valentine杀白细胞素(PVL)基因的金黄色葡萄球菌。方法收集我院2005年1月至2006年1月从临床多种标本中分离的金黄色葡萄球菌,用多重PCR同时检测葡萄球菌16S rRNA基因、mecA基因和lukS／F-PV基因。多重PCR检测MRSA的SCCmec基因型及亚型。结果195株金黄色葡萄球菌经多重PCR检测,121株为耐甲氧西林金黄色葡萄球菌(MRSA),74株为甲氧西林敏感金黄色葡萄球菌(MSSA),共检测到26株金黄色葡萄球菌lukS／F-PV基因阳性,阳性率为13．3％(26／195)。其中19株为MRSA,阳性率为15．7％(19／121);7株为MSSA,阳性率为12．2％(7／74)。19株lukS／F-PV基因阳性的MRSA的SCCmec基因型分别为SCCmecIII型10株、SCCcecIIIA型4株、SCCmecIV型4株及SCCmec I型1株。26株lukS/F-PV基因阳性的分离株有11株分离自脓液或创面分泌物,10株分离自痰标本,3株分离自血液标本,2株分离自尿液。结论在温州地区分离的MRSA和MSSA中都能检测到Panton-Vatentine杀白细胞素基因,含Panton-Valentine杀白细胞素基因MRSA的SCCmec基因型主要为SCCmecⅢ,含PVL基因的金黄色葡萄球菌主要引起化脓性感染和肺部感染。

引用

页码：1112 / 1115

页数：4

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