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利用cDNA-AFLP技术鉴定甘蓝显性核不育基因相关表达序列

被引：14
作者：
娄平
王晓武
Guusje.Bonnema
方智远
机构：
[1] 中国农业科学院蔬菜花卉研究所
[2] Laboratory of Plant Breeding
[3] Wageningen University
[4] Wageningen AJ
[5] Netherlards
[6] 中国农业科学院蔬菜花卉研究所 北京
[7] 北京
[8] 北京
来源：
园艺学报 | 2003年 / 06期
关键词：
甘蓝; 显性核不育; cDNA-AFLP; 差异表达序列;
D O I：
10.16420/j.issn.0513-353x.2003.06.007
中图分类号：
S635 [甘蓝类];
学科分类号：
摘要：
利用cDNA-AFLP技术,比较分析了一个甘蓝与两个青花菜自交系回交转育的显性核基因雄性不育材料与对应可育亲本植株花蕾发育过程中基因表达的差异。将花蕾混合提取RNA合成cDNA建立6个cDNA池,分析128个引物组合获得了26000个片段,共鉴定24个片段与雄性不育相关,其中13条表现为质的差异,11条表现为量的差异;将其中引物组合A16T15产生的300 bp左右的差异片段进行克隆测序,BLAST结果表明,该序列与花粉特异性相关的硫氧还蛋白氨基酸序列同源性达89％。
引用
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页码：668 / 672
页数：5
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