高压氧对持续局灶性脑缺血细胞凋亡的影响及其作用机制

被引:25
作者
吴伟
汪洋
邱阳
机构
[1] 中国医科大学附属第一医院急诊科
[2] 中国医科大学附属第二医院二部 沈阳
[3] 沈阳
关键词
局灶脑缺血; 细胞凋亡; 高压氧治疗;
D O I
暂无
中图分类号
R743 [脑血管疾病];
学科分类号
1002 ;
摘要
目的 探讨高压氧(HBO)对持续性局灶性脑缺血细胞凋亡的影响及其作用机制。方法 选择4~5个月龄SD大鼠65只,随机分为缺血组、HBO组和假手术组。观察脑梗死灶体积、凋亡细胞数量及凋亡调控基因蛋白表达的变化。结果 HBO组与缺血组相比,6 h~10 d各相应时间点梗死灶体积均明显缩小,差异均有极显著性意义(P均<0.01);HBO 组缺血48 h后,TUNEL 阳性凋亡细胞数较缺血组明显减少(P<0.01);HBO 组与缺血组相比,在缺血6 h~10 d各时间点 Bcl-2阳性细胞增加;差异有显著性意义;Bax阳性细胞在缺血6 h~10 d各时间点均减少,差异有显著性意义。结论 脑缺血早期暴露于高压氧可以减少局灶性脑缺血梗死灶体积;高压氧能抑制脑缺血细胞凋亡,其作用与上调Bcl-2蛋白、下调Bax蛋白表达有关。
引用
收藏
页码:14 / 17
页数:4
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