转化生长因子β1对系膜细胞纤溶酶原激活物抑制物1表达的影响

被引：9

作者：

姜宗培

余学清

陈雄辉

杨念生

河宪珠

李熙渤

机构：

[1] 中山大学附属第一医院肾内科

[2] 韩国汉城Soon Chun Hyang大学玄岩肾脏研究所

[3] 韩国汉城Soon Chun Hyang大学玄岩肾脏研究所广州

[4] 广州

来源：

中华肾脏病杂志 | 2004年 / 04期

关键词：

转化生长因子β; 纤溶酶原激活物抑制物; 反应性氧基; 系膜细胞;

D O I：

暂无

中图分类号：

R692 [肾疾病];

学科分类号：

1002 ; 100210 ;

摘要：

目的观察转化生长因子(TGF)β1对肾小球系膜细胞(GMC)纤溶酶原激活物抑制物(PAI)-1表达的影响,并探讨反应性氧基(ROS)在TGF-β1诱导的PAI-1表达中的作用。方法体外培养大鼠GMC,分别用TGF-β1(2ng/ml)和葡萄糖氧化酶(GO)(10mU/ml)刺激,并用BSO和抗氧化剂N-乙酰半胱氨酸(NAC)进行干预处理。采用Western印迹检测PAI-1蛋白表达;RT-PCR和Northern杂交检测PAI-1mRNA表达;合成的荧光素纤溶酶底物测定纤溶酶活性。结果外源性TGF-β1和GO可显著上调大鼠系膜细胞PAI-1蛋白和mRNA的表达并降低纤溶酶活性。BSO可显著增强TGF-β1和GO诱导的系膜细胞PAI-1mRNA的表达;而NAC可显著地逆转由TGF-β1和GO诱导的PAI-1mRNA表达的上调作用。结论TGF-β1可显著上调系膜细胞PAI-1的表达并抑制纤溶酶活性。ROS在TGF-β1诱导的系膜细胞PAI-1表达上调的信号传递途径中可能起了信号传递分子的作用。

引用

页码：26 / 29

页数：4

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