大鼠脑缺血再灌组织中补体沉积及降纤酶干预的实验观察

被引：9

作者：

罗晓光

李绍英

曲恒春

刘亢丁

苏志强

机构：

[1] 中国医科大学附属第一临床医院神经内科!沈阳

[2] 不详

[3] 中国医科大学附属第一临床医院胸科实验室

[4] 白求恩医科大学附属第一临床医院神经内科

[5] 哈滨医科大学附一院神经内科

来源：

中国神经免疫学和神经病学杂志 | 1998年 / 04期

关键词：

脑缺血; 缺血再灌损伤; 补体; 蛇毒;

D O I：

暂无

中图分类号：

R743.32 [脑血栓形成];

学科分类号：

摘要：

目的本实验旨在观察脑缺血再灌损伤中是否有补体参与，其参与是否与组织坏死有关，蛇毒制剂降纤酶对其有无干预作用。方法制成大鼠大脑中动脉缺血2h再灌22h动物模型，运用免疫组化方法检测再灌组织中的补体沉积，并给予降纤酶处理，观察其干预效果。结果补体C3在缺血再灌组织中大量沉积，为强阳性；降纤酶干预后补体沉积减少，为弱阳性；正常组及假手术组脑组织中无补体沉积。经X2检验证实补体沉积与组织坏死关系密切（P＜0．01）。给药组白细胞浸润较未给药组明显减少（P＜0．05），组织损伤程度也相对较轻。结论脑缺血再灌组织中有大量补体参与，补体的参与同组织坏死有密切关系。蛇毒制剂降纤酶可减少缺血再灌组织中补体的沉积，从而削减白细胞浸润，减轻脑组织损伤程度。

引用

页码：203 / 208

页数：6

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