大鼠脑缺血再灌注半暗带β淀粉样前蛋白mRNA表达与梗死体积的关系

被引：6

作者：

陈元新

林祥通

赵刚

机构：

[1] 复旦大学附属华山医院核医学科,复旦大学附属华山医院核医学科,中西医结合神经病研究所上海

来源：

中国神经科学杂志 | 2002年 / 01期

关键词：

脑缺血再灌注; 半暗带; β淀粉样前蛋白; 转录;

D O I：

暂无

中图分类号：

R743 [脑血管疾病];

学科分类号：

1002 ;

摘要：

为了探讨大鼠局灶性脑缺血再灌注缺血半暗带β淀粉样前蛋白 (APP)转录水平与缺血时间及梗死体积的相互关系 ,用插线法建立大鼠局灶性脑缺血再灌注模型 ,剥取缺血半暗带皮质组织 ,采用半定量逆转录聚合酶链式反应 (RT PCR) ,测定永久性缺血 4 8h和不同缺血时间再灌注 4 8h后 ,APPmRNA水平的变化。结果显示 ,梗死体积随再灌注前缺血时间的延长而增大 ,皮质半暗带缺血 30min再灌注 4 8hAPPmRNA表达升高 ;缺血 6 0min和缺血 12 0min再灌注 4 8hAPPmRNA升高明显 ;缺血 180min再灌注 4 8h和永久性缺血 4 8hAPPmRNA达到高峰。提示缺血半暗带APPmRNA的表达随再灌注前缺血时间延长而增加并与梗死体积有一定的相关性 ,早期再灌注可减少其表达。 ((GFDA1) )

引用

页码：454 / 457

页数：4

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