二恶英诱发斑马鱼初期胚的循环系统障碍

被引：10

作者：

董武

魏强

寺冈宏树

平贺武夫

机构：

[1] 酪农学园大学兽医毒性学教室

[2] 中国医学科学院实验动物研究所

来源：

中国实验动物学报 | 2002年 / 02期

关键词：

TCDD; 斑马鱼; 胚胎; CYP1A; 心血管系统;

D O I：

暂无

中图分类号：

X503.225 [鱼类、水产];

学科分类号：

摘要：

目的　观察二恶英 (2 ,3,7,8 tetrachlorodibenzo p dioxin ,以下略为TCDD)对斑马鱼 (Zebrafish)初期胚胎的毒性作用。方法　斑马鱼胚从受精后 2 4h直至观察放在 2 4孔培养皿内水浴染毒。采用形态学、组织学观察法及细胞色素P4 5 0 1A(CYP1A)抗体染色法。结果　 0 1μg LTCDD未观察到明显的变化 ,但是 0 3～ 10 μg L浓度的TCDD染毒时 ,首先观察到后主静脉的血流减缓与停滞为共同特征的毒性症状。在染毒群同时还观察到心囊、卵黄囊、头部、躯体等不同程度的水肿以及头部的畸形。在 180h ,摄食对照群 ,0 1μg L、0 3μg L、0 5 μg L、1μg L和 10 μg L各自的死亡率分别是 0、0、5 %、6 0 2 %、6 1 8%、10 0 %。CYP1A抗体染色发现对照群无阳性反应 ,而染毒群在血管上皮看到极强的阳性反应。结论　本试验表明TCDD对斑马鱼胚胎的循环系统有极强的损坏作用 ,而且这种损坏与CYP1A相关联

引用

页码：7 / 10

页数：4

共 12 条

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