远程缺血后适应对脑缺血损伤的实验研究

被引：5

作者：

宋兆晶 ^{[1
]}

牛小媛 ^{[1
]}

吉训明 ^{[2
]}

罗玉敏 ^{[2
]}

机构：

[1] 山西医科大学第一临床医院

[2] 首都医科大学宣武医院

来源：

中国医学创新 | 2010年 / 7卷 / 11期

关键词：

脑缺血; 缺血/再灌注损伤; 远程缺血后适应; Caspase-3;

D O I：

暂无

中图分类号：

R743 [脑血管疾病];

学科分类号：

摘要：

目的探讨远程缺血后适应对大鼠大脑中动脉缺血2 h再灌注24 h脑梗死体积、神经功能评分及Caspase-3表达的影响。方法大鼠脑缺血模型采用线栓法制备。缺血SD大鼠(280～300 g)48只,随机分为假手术组(n=6)、对照组(即单纯脑缺血/再灌注损伤,n=14)和远程缺血后适应组(脑缺血后给予双下肢的非致死性的缺血再灌注)。根据后适应时间不同,后组者又分为缺血即刻后适应组(n=14)和再灌注即刻后适应组(n=14)。于大脑中动脉再灌注24 h进行神经功能评分、脑组织梗死体积以及Caspase-3表达的测定。脑组织梗死体积采用四氮唑红(TTC)染色法测定。Caspase-3的表达应用免疫组织化学法检测。结果远程缺血后适应组的脑梗死体积与单纯缺血组相比明显降低,差异有显著性(P<0.05);与假手术组相比,单纯缺血组Caspase-3阳性细胞数明显增多,差异有显著性(P<0.05);与对照组比较,缺血即刻后适应组Caspase-3阳性细胞数降低,差异有显著性(P<0.05)。结论远程缺血后适应能减轻脑缺血/再灌注损伤,其保护作用可能与降低Caspase-3的表达有关。

引用

页码：1 / 4

页数：4

共 11 条

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