根癌农杆菌介导的香蕉枯萎病菌4号生理小种的转化

被引：17

作者：

李敏慧 ^{[1
]}

张荣 ^{[1
,2
]}

姜大刚 ^{[3
]}

习平根 ^{[1
]}

庄楚雄 ^{[3
]}

姜子德 ^{[1
]}

机构：

[1] 不详

[2] 华南农业大学资源与环境学院

[3] 不详

[4] 华南农业大学园艺学院

[5] 华南农业大学生命科学院

[6] 不详

来源：

植物病理学报 | 2009年 / 04期

基金：

广东省自然科学基金;

关键词：

香蕉枯萎病菌4号生理小种; 农杆菌介导的转化; 影响因子优化;

D O I：

10.13926/j.cnki.apps.2009.04.015

中图分类号：

S436.68 [多年生草本果类病虫害];

学科分类号：

摘要：

本文针对香蕉枯萎病菌4号生理小种这一对我国香蕉生产构成了极大威胁的检疫性有害生物,建立了根癌农杆菌介导的该生理小种的转化体系,确定了影响转化效率主要因子的最佳条件分别是:共培养乙酰丁香酮(AS)浓度为200μmol/L、共培养时间为48 h、培养温度为25℃、诱导培养基pH值为5.5。此条件下,转化效率达到21～24个转化子/104香蕉枯萎病菌孢子。PCR和Southern杂交分析表明外源的T-DNA已经成功随机地整合到该病原菌基因组中,且多为单拷贝。应用该转化体系已获得近25 000个转化子,为研究该生理小种致病机制奠定了基础。

引用

页码：405 / 412

页数：8

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