The nitrogen loss pathway in winter wheat and summer maize rotation system was studied based on field experimental data. The results showed that nitrogen recovery rate was significantly decreased with nitrogen fertilization rate increased, while residual rate and losses rate had an increasing trend. Accumulated ammonia volatilization loss in winter wheat and summer maize rotation was 12. 8(N0), 22.0(N120), 33. 0(N240) and 64. 5 kg N ha-1 (N360) respectively and rate of ammonia volatilization loss was 3.8, 4.2 and 7.2% respectively while urea was mixed with 0 -10 cm soil or spread before irrigation. Denitrification loss with acetylene-soil core incubation method in winter wheat was lower than 1 kg N ha-1 and rate of denitrification loss was 0. 21 - 0. 26% or trace. Denitrification loss in summer maize was 1 - 14 kg N ha-1 and rate of denitrification loss was 1-5%. The total gaseous loss in winter wheat and summer maize rotation system was less than 10%, and the main nitrogen fertilizer loss way was leach
