液质联用法筛选黄芩中清除自由基的活性物质

被引：14

作者：

刘渊宏 ^{[1
]}

宋龙 ^{[2
]}

机构：

[1] 甘肃奇正藏药有限公司

[2] 上海中医药大学中药学院

来源：

中国中医基础医学杂志 | 2015年 / 21卷 / 09期

关键词：

自由基; 黄芩; 液质联用;

D O I：

暂无

中图分类号：

R284 [中药化学];

学科分类号：

100804 [中药化学];

摘要：

目的:用液质联用方法快速筛选黄芩药材中用于清除人体内自由基的活性物质。方法:通过对黄芩中提取制得含4种单体(野黄芩苷、黄芩苷、野黄芩素、黄芩素)的样品溶液,分别与各自由基制得的溶液混合,以甲醇为对照孵化培养,液相色谱-质谱联用方法对黄芩提取液中各组分分离,以峰面积的变化来进行考察。结果:清除自由基实验结果显示,这4种成分经过与超氧自由基、羟基自由基、过氧自由基反应后含量都有不同程度的减少。结论:黄芩中4种成分对DPPH、超氧自由基、羟基自由基和过氧自由基都具有一定的清除作用。

引用

页码：1156 / 1159

页数：4

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