3种不同方法检测艰难梭菌芽孢率的比较

被引:7
作者
徐蓉
葛平
陈蓉
刘学杰
机构
[1] 上海市临床检验中心
关键词
芽孢; 艰难梭菌; 检测;
D O I
暂无
中图分类号
R446.5 [微生物学检验];
学科分类号
摘要
目的了解艰难梭菌检测的芽孢率并进行比较。方法采用平板涂布法、相差显微镜计数法和孔雀绿染色法3种检测方法对艰难梭菌ATCC 43596和VPI 10463经培养96 h和120 h后的芽孢率进行了比较分析。结果显示平板涂布法芽孢检出率最高为(25.3%、15.6%),变异较大(SD=7.1%,4.3%);相差显微镜计数法和孔雀绿染色法结果相近分别为(13.3%、9.4%)、(13.4%、9.8%),变异较小(SD=1.9%、0.8%)、(SD=1.4%、0.9%)。结论在选用平板涂布法检测芽孢率时,建议联合相差显微镜法或者孔雀绿染色法以减少偏移。
引用
收藏
页码:201 / 202+205 +205
页数:3
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