PCR-DGGE技术在农田土壤微生物多样性研究中的应用

被引：82

作者：

罗海峰

齐鸿雁

薛凯

张洪勋

机构：

[1] 中国科学院生态环境研究中心环境生物技术研究室

来源：

生态学报 | 2003年 / 08期

关键词：

变性梯度凝胶电泳(DGGE); 基因组DNA; 16SrRNA; 微生物多样性;

D O I：

暂无

中图分类号：

S154.3 [土壤微生物学];

学科分类号：

摘要：

变性梯度凝胶电泳技术 ( DGGE)在微生物生态学领域有着广泛的应用。研究采用化学裂解法直接提取出不同农田土壤微生物基因组 DNA,并以此基因组 DNA为模板 ,选择特异性引物 F357GC和 R518对 1 6Sr RNA基因的 V3区进行扩增 ,长约 2 30 bp的 PCR产物经变性梯度凝胶电泳 ( DGGE)进行分离后 ,得到不同数目且分离效果较好的电泳条带。结果说明 ,DGGE能够对土壤样品中的不同微生物的 1 6Sr RNA基因的 V3区的 DNA扩增片断进行分离 ,为这些 DNA片断的定性和鉴定提供了条件。与传统的平板培养方法相比 ,变性梯度凝胶电泳 ( DGGE)技术能够更精确的反映出土壤微生物多样性 ,它是一种有效的微生物多样性研究技术。

引用

页码：1570 / 1575

页数：6

共 14 条

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