一株产木聚糖酶菌株的分离、鉴定及其酶学特性研究

被引：12

作者：

孙振涛 ^{[1
]}

刘建军 ^{[1
]}

赵祥颖 ^{[2
]}

杜金华 ^{[1
]}

黄伟红 ^{[1
]}

机构：

[1] 山东农业大学生命科学学院

[2] 山东食品发酵工程研究设计院

来源：

生物技术 | 2007年 / 04期

关键词：

木聚糖酶; 分离; 鉴定; 酶学性质;

D O I：

10.16519/j.cnki.1004-311x.2007.04.016

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

以木聚糖为唯一碳源,采用平板水解圈筛选和摇瓶发酵相结合的方法,从土壤中分离、筛选到一株产木聚糖酶的细菌xy-7,根据其形态和生理、生化特性,并结合16S rDNA序列分析,初步鉴定为坎皮纳斯类芽孢杆菌(Paenibacillus campinasensis)。经测定,xy-7所产木聚糖酶的最适作用温度为60℃,最适作用pH为7.0。该酶热稳定性较好,60℃时保温2h酶活保持为原来的73%。此外,该酶的pH作用范围较广,pH 9.0时酶活仍能保持68%,属于耐碱性木聚糖酶。这些性质表明,该酶在制浆造纸等行业具有较好的应用前景。

引用

页码：74 / 77

页数：4

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